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鉴于在治疗时平行测定在血清和细胞内的药物浓度十分重要,同时,为了在治疗前和治疗时检测单独用药或合并用药的效果,有必要发展快速敏感的检定方法。本文报道酶联免疫吸附法(ELISA)快速测定HSV-1、2型对抗病毒药物的敏感性。检测条件生长在24孔板上的人胚肺纤维母细胞(HL)以不同病毒量的HSV-1感染,1～7天后吸取上清液,细胞加800μl 蒸馏水冻融再以4.5%NaCl 使成等渗,细胞内容物和先吸取的上清液分别在用豚鼠抗-HSV IgG 包被的微孔
In view of the importance of parallel determination of drug concentrations in serum and cells at the time of treatment, in the meantime, it is necessary to develop a rapid and sensitive assay in order to detect the effects of drug administration alone or in combination before and during treatment. This article reports the enzyme-linked immunosorbent assay (ELISA) rapid determination of HSV-1,2 type of anti-viral drug sensitivity. Detection conditions Human embryonic lung fibroblasts (HL) grown on 24-well plates were infected with different viral loads of HSV-1 and supernatants were aspirated after 1 to 7 days. Cells were lysed and thawed with 800 μl of distilled water and then incubated with 4.5% NaCl Isotonic, cell contents and supernatant aspirated were separately incubated in guinea pig anti-HSV IgG-coated micropores