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本文介绍基于在流体中玻璃碳电极上的电化学氧化作用,用流动注入法连续测定盐酸肼苯哒嗪。方法:用往复泵使流动相Walpole 醋酸盐缓冲液-无水甲醇(40∶60)流经一Metrohm EA-1096型电化学池(具E611型检测器),流速:1.0ml/min。池中配有玻璃碳工作电极、银-氯化银参比电极与另一玻璃碳辅助电极。用微量注射器将样品注入进样口,进样量:50μl。整个系统用HPLC 不锈钢管(0.02cm)连接固定,在室温下用记录器记录电流。标准、样品液制备:用Walpole 醋酸盐缓冲液(pH4.2)配制盐酸肼苯哒嗪贮备液(0.1mg/ml),再将其稀释成1～50μg/ml 的标准液,用作制备工作曲线。
This article describes the continuous determination of hydralazine hydrochloride by flow injection based on the electrochemical oxidation of glassy carbon electrodes in a fluid. Method: The mobile phase Walpole acetate buffer-anhydrous methanol (40:60) was passed through a Metrohm EA-1096 electrochemical cell (with E611 detector) using a reciprocating pump at a flow rate of 1.0 ml / min. The cell is equipped with a glassy carbon working electrode, a silver-silver chloride reference electrode and another glassy carbon auxiliary electrode. The sample is injected into the injection port using a micro syringe and the injection volume is 50 μl. The whole system was connected and fixed with HPLC stainless steel tube (0.02cm), and the current was recorded by the recorder at room temperature. Standard, Sample Preparation: A stock solution of hydralazine hydrochloride (0.1 mg / ml) was prepared in Walpole acetate buffer (pH 4.2) and diluted to a standard solution of 1 to 50 μg / ml for preparation Working curve.