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目的:研究莪芪抗瘤方对二乙基亚硝胺(DEN)诱导大鼠肝癌过程中相关基因蛋白阳性细胞数的影响。方法:建立DEN诱导大鼠肝癌模型,分别于诱导第12周末和第16周末采用免疫组化染色方法检测莪芪抗瘤方对大鼠肝细胞增殖细胞核抗原(PCNA)、抗癌基因(p53)蛋白阳性表达的影响。结果:p53阳性细胞可见于癌前肝细胞增生结节中;莪芪抗瘤方在诱导第12周末和第16周末均能明显抑制p53的阳性表达(P<0.05),显著降低PCNA(P<0.01)。结论:在DEN诱导大鼠肝癌模型过程中,于肝癌癌前病变时p53即发生了基因突变、参与肝癌的形成。莪芪抗瘤方可抑制大鼠诱癌过程中肝细胞的增殖活性和p53的突变,从而延缓肝癌的形成。
Objective: To study the effect of Curcuma Anticancer Decoction on the number of related protein positive cells in rat hepatocellular carcinoma induced by diethylnitrosamine (DEN). Methods: The model of hepatocellular carcinoma induced by DEN was established in rats. Immunohistochemical staining was used to detect the expression of proliferating cell nuclear antigen (PCNA) and anti-oncogene (p53) in rat hepatocytes at the 12th week and the 16th week respectively. Protein positive expression. Results: p53 positive cells were found in the premalignant hepatocyte hyperplasia nodules. Curcuma Qi’ang’ao’antang could significantly inhibit the expression of p53 at the 12th week and the 16th weekend (P <0.05), and significantly decrease the PCNA (P < 0.01). Conclusion: In the process of DEN-induced rat hepatocellular carcinoma, p53 is a gene mutation in the precancerous lesions of liver cancer, which is involved in the formation of hepatocellular carcinoma. Curcuma anticoagulant can inhibit the growth of rat hepatoma cells during the induction of cancer activity and p53 mutations, thereby delaying the formation of liver cancer.