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目的探讨血清总胆汁酸(TBA)作为山豆根致肝损伤早期敏感标志物的可能性。方法取Wistar大鼠,随机分为对照组和山豆根水煎液组(简称山豆根组),山豆根组每天灌胃给予水煎液6 g·kg-1,对照组灌服蒸馏水,于给药1 d、7 d、16 d分批处理动物。测定常规血液生化指标—丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TB)、碱性磷酸酶(ALP)、总蛋白(TP)、白蛋白(ALB)和TBA的水平;计算肝脏指数,并进行HE染色,观察肝组织病理形态学变化。另取正常动物,测定肝、肾、脾、肺、心、脑、胸腺组织中TBA的含量。结果与同时间点对照组相比,山豆根组常规生化指标ALT表现出良好的时效关系,于给药7 d、16 d升高(P<0.01),AST于给药1 d升高(P<0.01),其他时间点无明显变化,肝脏指数和肝组织病理形态学检查无明显变化。TBA于给药1 d、16 d升高(P<0.01),变化较ALT早,与同时间点对照组相比,TBA数值的变化倍数高于ALT和AST。组织含量测定显示肝组织中TBA含量较高。结论 TBA可作为山豆根致肝损伤的早期敏感生物标志物之一。
Objective To investigate the possibility of using serum total bile acid (TBA) as early sensitive marker of liver injury induced by shadoung root. Methods Wistar rats were randomly divided into control group and Shandou root decoction group (Shanduo root group). Shandou root group was given ganoderma decoction 6 g · kg-1 orally everyday. The control group was given distilled water Animals were treated in batches on day 1, day 7 and day 16 after administration. The serum levels of ALT, AST, TB, ALP, TP, (ALB) and TBA. Liver index was calculated and HE staining was performed to observe the pathological changes of liver tissue. Another normal animal, liver, kidney, spleen, lung, heart, brain, thymus TBA content. Results Compared with the control group at the same time point, the ALT showed a good effect of aging on the 7th day and the 16th day after administration (P <0.01), while AST increased on the 1st day P <0.01). There was no significant change at other time points. There was no significant change in liver index and histopathological examination of liver tissue. TBA increased on the 1st day and the 16th day after administration (P <0.01), which was earlier than ALT. The change of TBA was higher than that of ALT and AST at the same time point. Tissue content measurement showed a high level of TBA in liver tissue. Conclusion TBA can be used as one of the early sensitive biomarkers of liver injury induced by shadouban.