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采用超声辅助提取法从马尾松花粉中提取总黄酮,通过单因素实验考察乙醇体积分数、提取时间、料液比以及提取次数等4个因素对马尾松花粉总黄酮得率的影响,并采用星点设计-效应面法优化总黄酮提取工艺。结果表明:马尾松花粉总黄酮的最佳提取工艺条件为乙醇体积分数62.5%,提取时间28.8 min,料液比1∶24,提取次数3次。在此优化条件下,马尾松花粉总黄酮的实际得率为1.37%,与理论值(1.32%)较为接近,表明采用星点设计-效应面法优化马尾松花粉总黄酮提取工艺的方法可行。
Total flavonoids were extracted from Masson pine pollen by ultrasonic-assisted extraction. The effects of ethanol concentration, extraction time, solid-liquid ratio and extraction times on the yield of total flavonoids of Masson Pine were studied by single factor experiments. Design Optimization - Response Surface Method for Extraction of Total Flavonoids. The results showed that the optimal extraction conditions for total flavonoids from Masson pine were 62.5% of ethanol, 28.8 min of extraction time, 1:24 of material to liquid ratio and 3 times of extraction. Under the optimum conditions, the actual yield of the total flavonoids of Pinus massoniana was 1.37%, which was close to the theoretical value of 1.32%. This indicated that it was feasible to optimize the extraction process of the total flavonoids of Pinus massoniana by using the star-point design-response surface method.