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目的测定46个不同产地种源草珊瑚叶、茎的生物量以及活性成分落新妇苷、迷迭香酸、异嗪皮啶含量,以总量为考查指标,筛选优良种源。方法样品来自福建三明三元吉口采育场草珊瑚种植栽培示范区3年生成熟草珊瑚,每个产地随机取20株,分叶与茎,晾干,称量,计算成每10株叶、茎干品重量作为生物量;含量测定采用HPLC。色谱柱:Ultimate C18(250 mm×4.6 mm,5μm);流动相A为乙腈-0.2%磷酸溶液(18∶82),B为乙腈-0.2%磷酸溶液(30∶70);梯度洗脱:0~30 min,A→B;流速:1 mL·min?1;柱温:室温;检测波长:茎部位(测定迷迭香酸、异嗪皮啶)为344 nm,叶部位(测定落新妇苷、迷迭香酸)为290 nm;分析有效成分含量、生物量以及总量。结果不同地理种源草珊瑚生物量差别显著,含量差别较大,计算总含量与总量均有较大差异,筛选5个高产质优的产地有3个来自福建三明。结论多指标有效成分总含量结合生物量筛选高产优质良种,指导科学种植有很大意义。
Objective To determine the biomass of leaves and stems and the contents of astilbin, rosmarinic acid and isoxabenazine in 46 species of provenances from different provenances. The total provenances were screened for excellent provenances. Methods The samples were from the coral cultivation demonstration area of Sikoukou, Sanming, Sanming, Fujian Province for three years to produce the mature coral. 20 plants were randomly selected from each producing area, divided into leaves and stems, dried, weighed and counted into 10 leaves and stems Dry weight as biomass; determination of content using HPLC. Column: Ultimate C18 (250 mm × 4.6 mm, 5 μm); mobile phase A was acetonitrile-0.2% phosphoric acid solution (18:82); B was acetonitrile-0.2% phosphoric acid solution ~ 30 min at A → B; flow rate: 1 mL · min -1; column temperature: room temperature; detection wavelength: 344 nm at the stem site (determined for rosmarinic acid, isoxabepilide) , Rosmarinic acid) was 290 nm; analysis of active ingredient content, biomass and total amount. Results The results showed that the biomass of grass coral differed significantly among different geographical provenance species. The contents of total biomass and total biomass were significantly different. Three of the five high yielding and quality producing sites were screened from Fujian Sanming. Conclusion The total content of multi-index active ingredients in combination with biomass screening of high-yielding high-quality elite, guiding scientific planting is of great significance.