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背景:应用酸性肽对血管型痴呆vasculardementia,VD小鼠模型进行()治疗研究,是否可以发现酸性肽的治疗效果。目的:研究酸性肽对血管型模型痴呆小鼠学习记忆能力和它们脑中蛋白质和一氧化氮含量的变化并与正常小鼠相比较。设计:随机对照的实验研究。单位:郑州大学医学院生物化学与分子生物学教研室。对象:实验于2002-10/2003-02在郑州大学医学院生物化学与分子生物学教研室的第一研究室和动物房进行。取昆明种小鼠84只,跳台实验低于正常小鼠平均反应时间的1只动物被排除。随机分为7组:正常组,模型组,盐水组,脑复康组,酸性肽高浓度组,酸性肽中浓度组,酸性肽低浓度组。方法:除12只正常组小鼠外,其余的动物一直用高脂乳剂05mL/d灌胃共10d,按照高维娟等的方法建立VD动物模型。模型组不作任何治疗,盐水组用生理盐,脑复康组应用脑复康,酸性肽高、中、低组应用不同浓度酸性肽治疗,口服给药治疗15d。之后用跳台试验进行学习记忆功能的变化测试,随后处死小鼠,测定脑中蛋白质和一氧化氮水平。主要观察指标:各组小鼠学习记忆功能和脑中蛋白质和一氧化氮水平。结果:①跳台试验的结果表明酸性肽能明显地减少痴呆小鼠跳台试验的错误次数,能显著地增加痴呆小鼠的学习记忆能力。②生化分析结果表明,模型组小鼠因脑缺血而使
BACKGROUND: Application of acidic peptides in the treatment of vascular dementia vascular dementia (VD) mouse model can find out the therapeutic effect of acidic peptides. OBJECTIVE: To study the changes of learning and memory abilities and the content of protein and nitric oxide in brain of vascular dementia model mice treated with acidic peptides and to compare with normal mice. Design: Randomized controlled experimental study. Unit: Department of Biochemistry and Molecular Biology, Zhengzhou University School of Medicine. PARTICIPANTS: The experiment was performed in the first laboratory and animal room of the Department of Biochemistry and Molecular Biology, Zhengzhou University Medical College from October 2002 to February 2003. A total of 84 Kunming mice were taken, and one animal whose jumping test was lower than the average response time of normal mice was excluded. Randomly divided into 7 groups: normal group, model group, saline group, Naofukang group, high concentration of acidic peptide group, acidic peptide concentration group, acidic peptide low concentration group. Methods: Except for 12 normal mice, the remaining animals were fed with high-fat emulsion (05mL / d) for 10 days. VD animal models were established according to Gao et al. The model group did not make any treatment. Saline group used physiologic salt, Naofukang group was treated with Naofukang, acid peptide was high, medium and low groups were treated with different concentration of acidic peptide, and oral administration for 15 days. Afterward, the change test of learning and memory function was conducted by jumping test, and then the mice were sacrificed to determine the brain protein and nitric oxide levels. MAIN OUTCOME MEASURES: Learning and memory function and protein and nitric oxide levels in brain of mice in each group. Results: (1) The results of jumping test showed that acidic peptides can significantly reduce the number of errors in jumping test of dementia mice, and significantly increase the learning and memory ability of dementia mice. ② biochemical analysis showed that model mice caused by cerebral ischemia