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目的研究糖尿病大鼠肾脏炎症因子RANTES的表达,并探讨厄贝沙坦对其保护作用的机制。方法 45只雄性Wistar大鼠行右肾切除术,术后两周,随机分成糖尿病模型组和正常对照组(A组),将糖尿病模型组大鼠随机分为糖尿病大鼠对照组(B组)和糖尿病大鼠治疗组(C组)。应用酶免疫分析法测定大鼠24h尿白蛋白排泄率,采用HE、PAS染色行肾脏组织形态学分析,用Real time FQ-PCR检测RANTES mRNA表达情况。结果与A组相比,B组大鼠UAER于第8周时即显著升高达14.00倍,16周时,UAER仍持续上升。C组与B组相比,第8周及16周UAER均有不同程度的下降,差异有统计学意义(P<0.05),但仍高A组(P<0.05);糖尿病组大鼠肾脏RAN-TESmRNA表达明显升高,与白蛋白排泄率、肾脏肥大指数呈正相关,厄贝沙坦组大鼠肾脏RANTES mRNA表达明显下调。结论糖尿病大鼠肾脏RANTES表达明显升高,厄贝沙坦对糖尿病大鼠的肾脏有保护作用,其机制可能与下调RANTES表达有关。
Objective To investigate the expression of renal inflammatory factor RANTES in diabetic rats and explore the mechanism of irbesartan’s protective effect on it. Methods 45 male Wistar rats underwent right nephrectomy. Two weeks after the operation, they were randomly divided into diabetic model group and normal control group (group A). The diabetic model rats were randomly divided into diabetic rats control group (group B) And diabetic rats treated group (C group). Urine albumin excretion rate was determined by enzyme immunoassay. Renal histomorphology was analyzed by HE and PAS staining. RANTES mRNA expression was detected by Real time FQ-PCR. Results Compared with group A, the UAER in group B increased significantly by 14.00 folds at the 8th week. UAER continued to increase at 16 weeks. Compared with group B, UAER in group C and group B decreased to some extent at 8 weeks and 16 weeks (P <0.05), but still higher in group A (P <0.05) -TES mRNA expression was significantly increased, and albumin excretion rate, renal hypertrophy index was positively correlated, irbesartan group rats kidney RANTES mRNA expression was significantly decreased. Conclusion The expression of RANTES in kidney of diabetic rats is obviously increased. Irbesartan has a protective effect on the kidney of diabetic rats, which may be related to the down-regulation of RANTES expression.