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在制作免疫吸附剂时,常采用CNBr活化琼脂糖的方法。此法虽较简便,偶联的蛋白质量(配基)较多。但也有不少缺点,如:(1)琼脂糖被CNBr活化后产生了带阳电荷的异脲基,从而导致对蛋白质的非特异性吸附;(2)抗原(抗体)被偶联后易脱落;(3)CNBr为剧毒,操作要严加防护等。我们采用了三氯三氮六环(Trichloro-s-triazine,简称TsT)作为活化剂来活化纤维素。此法具有操作简便、偶联配基时pH范围广、毒性较小、试剂均有国产货源等优点。材料与方法1.三氯三氮六环(Aldrich,沪、津也有生产)
In the production of immunosorbent, CNBr activated agarose often used. Although this method is relatively simple, coupled protein quality (ligand) more. However, there are also many disadvantages, such as: (1) Sepharose is activated by CNBr to produce a positively charged isoureido, resulting in non-specific adsorption of proteins; (2) antigen (antibody) (3) CNBr is highly toxic, the operation should be strictly protected. We use trichloro-s-triazine (TsT) as an activator to activate cellulose. This method has the advantages of simple operation, wide pH range when coupled with ligand, less toxicity and domestic sources of reagents. Materials and methods 1. Trichloro triazacyclohexyl (Aldrich, Shanghai and Tianjin are also produced)