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目的:探讨巯基氧化还原试剂对葡萄糖刺激胰岛素分泌(glucose-stimulated insulin secretion,GSIS)影响,进而揭示其调节胰岛素分泌的可能机制。方法 :INS-l细胞经传代培养3~4 d后在KRBH液中,37℃培养箱孵育30 min,再用含有不同浓度葡萄糖和巯基氧化还原试剂的KRBH液中培养60 min。然后留取上清液进行胰岛素测定。结果:(1)INS-1细胞在2.5、5、10、15、20 mmol/L葡萄糖浓度范围内胰岛素分泌量逐渐增加,G5、G10、G15组间两两相比均有统计学意义(P<0.05);(2)与G10组相比,G10+DTBNP、G10+DTDP组胰岛素分泌量显著增加(P<0.05),且该效应可以被DTT所消除。(3)DTBNP、DTDP均能增加NIF处理组胰岛素分泌,但其增加幅度低于非NIF处理组(P<0.05);(4)与非DIA组相比,G10+DIA+DTBNP、G10+DIA+DTDP组胰岛素分泌增加幅度显著减低(P<0.05);(5)同G10组比较,G10+DIA+NIF+DTBNP、G10+DIA+NIF+DTDP组胰岛素分泌值增加(P<0.05)。结论 :本研究显示巯基氧化还原试剂对GSIS产生调节作用。DTDP、DTBNP可能通过对K_ATP、L型Ca_V通道及IP_3受体活性的调节,促进胰岛素分泌。
Objective: To investigate the effect of sulfhydryl redox reagent on glucose-stimulated insulin secretion (GSIS), and to reveal its possible mechanism of regulating insulin secretion. METHODS: INS-1 cells were subcultured for 3 to 4 days and then incubated in KRBH solution at 37 ° C for 30 min in a KRBH solution containing different concentrations of glucose and sulfhydryl redox reagent for 60 min. The supernatant is then taken for insulin determination. Results: (1) Insulin secretion in INS-1 cells gradually increased in the range of glucose concentration of 2.5, 5, 10, 15 and 20 mmol / L, and there was a significant difference between G5, G10 and G15 groups <0.05). (2) Compared with G10 group, insulin secretion of G10 + DTBNP and G10 + DTDP group increased significantly (P <0.05), and the effect could be eliminated by DTT. (3) Both DTBNP and DTDP increased the insulin secretion in NIF-treated group, but the increase was lower than that in non-NIF-treated group (P <0.05). (4) Compared with non-DIA group, (P <0.05). (5) Compared with G10 group, insulin secretion increased in G10 + DIA + NIF + DTBNP and G10 + DIA + NIF + DTDP groups (P <0.05). Conclusion: This study shows that sulfhydryl redox reagent regulates GSIS. DTDP and DTBNP may promote insulin secretion by regulating the activity of K_ATP, L-type Ca_V channel and IP_3 receptor.