论文部分内容阅读
目的探讨依达拉奉(edaravone)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的C57BL/6J帕金森病模型小鼠的保护作用及相关机制。方法 90只雄性C57BL/6J小鼠随机分为依达拉奉组(ED组)、帕金森病模型组(PD组)和生理盐水对照组(NS组),每组30只。ED组和PD组小鼠给予皮下注射MPTP建立PD模型后,ED组给予依达拉奉(3mg/kg)治疗。用滚轴实验检测小鼠的旋转次数,用免疫组织化学实验检测小鼠黑质酪氨酸羟化酶(TH)的表达,用RT-PCR和免疫印迹实验分别检测小鼠黑质脑源性神经营养因子(BDNF)mRNA与蛋白的表达。结果与NS组比较,ED组、PD组小鼠在滚轴实验中的旋转次数下降(P<0.05,P<0.01),黑质TH表达减少(P<0.05,P<0.01),黑质BDNF的mRNA(P均<0.01)和蛋白(P均<0.05)表达降低;与PD组比较,ED组小鼠在滚轴实验中的旋转次数增加(P<0.05),黑质TH表达增加(P<0.05),黑质BDNF的mRNA(P<0.01)和蛋白(P<0.05)表达升高。结论依达拉奉可增加C57BL/6JPD模型小鼠黑质区BDNF的mRNA及蛋白表达,降低MPTP对小鼠黑质的损伤,对多巴胺能神经元有保护作用。
Objective To investigate the protective effect of edaravone on mouse models of C57BL / 6J Parkinson’s disease induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mechanism. Methods Ninety male C57BL / 6J mice were randomly divided into edaravone (ED) group, PD model group (PD group) and NS control group (NS group), 30 rats in each group. ED and PD mice were given subcutaneous injection of MPTP to establish PD model, ED group were given edaravone (3mg / kg) treatment. The number of rotations of the mice was measured by a roller test, and the tyrosine hydroxylase (TH) expression in substantia nigra of the mice was detected by immunohistochemical staining. The brain-derived brain derived from substantia nigra was detected by RT-PCR and Western blotting Neurotrophic factor (BDNF) mRNA and protein expression. Results Compared with the NS group, the number of rotations of the mice in ED group and PD group decreased (P <0.05, P <0.01), the expression of TH in substantia nigra decreased (P <0.05, P <0.01) (P <0.01) and protein (P <0.05). Compared with the PD group, the number of rotations of the mice in the ED group increased (P <0.05) and the expression of TH <0.05), BDNF mRNA (P <0.01) and protein (P <0.05) increased in substantia nigra. Conclusion Edaravone can increase BDNF mRNA and protein expression in the substantia nigra of C57BL / 6JPD mice, decrease the damage of MPTP to substantia nigra of mice, and protect dopaminergic neurons.