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旨在敲除小麦赤霉菌(Fusarium graminearum) FGSG_08948基因,确定其缺失突变体表型,从而分析该基因的生物学功能.应用Split-Marker技术敲除FGSG_08948基因,构建含有潮霉素抗性基因hph的基因敲除盒,利用PEG介导法进行原生质体转化.在含有潮霉素的培养基上筛选转化子,通过PCR正负筛选,得到2个确定的敲除突变体,分别命名为△FGSG_08948 1-1、△FGSG_08948 1-2.表型观察发现:敲除突变体的菌落形态无明显差别,菌落生长速度略微滞后,孢子形态无差别,致病力无减弱,但产孢量有所上升.因此, FGSG_08948基因可能与小麦赤霉菌分生孢子的生长能力有关.“,”The purpose of this experiment was to knock out FGSG_08948 gene in Fusarium graminearum and to determine the phenotype of its deletion mutant, thereby analyzing the biological function of the gene. The FGSG_08948 gene was knocked out by Split-Marker method, and gene deletion cassette containing hygromycin phosphotransferase gene hph was constructed and was transformed into protoplast of wild type PH-1 by PEGmediated transformation method. Transformants were selected through medium containing hygromycin and then two deletion mutants were confirmed by PCR positive and negative screening, named as △FGSG_08948 1-1 and △FGSG_08948 1-2, respectively. The phenotype observation found that there was no significant difference between deletion mutant and wild type in colony morphology, spore morphology and pathogenicity. Colony growth rate of FGSG_08948 deletion mutant was slightly delayed. However, the spore morphology was not different, the pathogenicity was not weakened, but the sporulation increased. Therefore, FGSG_08948 gene might be related to spore formation in Fusarium graminearum.