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目的:探讨紫金莲醇提物2种萃取部位对甲醛致痛大鼠模型的镇痛作用及机制。方法:56只SD大鼠随机分6组,每组8只,分别设为正常组,模型组,紫金莲醇提物乙酸乙酯部位高(乙高组30 mg·kg-1)、低(乙低组15 mg·kg-1)剂量组,紫金莲醇提物正丁醇部位高(正高组,15 mg·kg-1)、低(正低组,8 mg·kg-1)剂量组。各给药组按剂量ig大鼠,给药7 d,每日1次。第7天末次给药40 min后,除正常组外,其余各组大鼠左后足跖sc 10%甲醛溶液50μL/只,建立大鼠甲醛致痛模型。观察紫金莲2种活性部位对甲醛致痛模型大鼠痛行为的影响,生化法检测大鼠血清谷氨酸和Ca2+含量,免疫组化法检测大鼠脑和脊髓c-fos阳性细胞表达和P物质(SP)含量。结果:与正常组比较,模型组大鼠表现出了典型的两时相痛行为学反应,且在脑和脊髓中检测到了大量的c-fos阳性细胞,P物质(SP),升高血清谷氨酸及Ca2+含量有显著性差异(P<0.05),表示造模成功。与模型组比较,紫金莲乙低组(15 mg·kg-1)和正低组(8 mg·kg-1),对大鼠第一时相伤害反应疼痛有显著的抑制作用(P<0.05),使大鼠血清谷氨酸和脑c-fos蛋白表达显著下降(P<0.05),血清Ca2+含量显著升高(P<0.05);紫金莲醇提物乙高组(30 mg·kg-1)和正高组(15 mg·kg-1)显著降低大鼠脊髓c-fos蛋白表达(P<0.05);紫金莲醇提物乙高、乙低组和紫金莲醇提物正高、正低组都能显著降低SP在大鼠脑和脊髓中的表达(P<0.05)。结论:紫金莲醇提物2种萃取部位对大鼠甲醛致痛模型均有镇痛作用,其镇痛机制可能与抑制体内谷氨酸的升高,抑制细胞内Ca2+浓度升高及降低c-fos,SP在中枢神经系统内的表达有关。
Objective: To investigate the analgesic effect and mechanism of two extracts of alcohol extract of Purpurin on rat model of formaldehyde-induced pain. Methods: Fifty-six SD rats were randomly divided into 6 groups (n = 8 each), which were set as normal group. The ethyl acetate fraction of ethanol extract of B group was higher (B group 30 mg · kg -1) (15mg · kg-1 for low dose group, 15mg · kg-1 for low dose group and 8mg · kg-1 for low dose group) . Each treatment group according to the dose ig rats, administered 7 d, 1 day. After the last administration on the 7th day for 40 min, except for the normal group, rats in each group were treated with formalin-induced formaldehyde pain model by 50 μL sc 10% formaldehyde solution in the left posterior plantar. To observe the effect of two active parts of Zizyphus jujubae to the pain behavior of rats induced by formaldehyde, the content of glutamate and Ca2 + in serum was detected by biochemistry method, the expression of c-fos positive cells in brain and spinal cord was detected by immunohistochemical method and P Substance (SP) content. Results: Compared with the normal group, the rats in the model group showed typical two-phase pain-induced behavioral responses. A large number of c-fos positive cells, substance P (SP), elevated serum valley There was a significant difference in the content of amino acid and Ca2 + (P <0.05), indicating successful modeling. Compared with the model group, the low-dose group (15 mg · kg -1) and the low-dose group (8 mg · kg -1) significantly inhibited the pain of the first phase injury in rats (P <0.05) (P <0.05), and the content of Ca2 + in serum was significantly increased (P <0.05). The content of serum glutamate and c-fos protein in rat serum was significantly decreased (P <0.05) ) And positive high group (15 mg · kg-1) significantly decreased the c-fos protein expression in spinal cord of rats (P <0.05) Can significantly reduce the expression of SP in rat brain and spinal cord (P <0.05). CONCLUSION: Both extract of ethanol extract of Hollyhock Linn on analgesic effect of formaldehyde induced pain model in rats, and its analgesic mechanism may inhibit the increase of glutamate in vivo, inhibit intracellular Ca2 + concentration and reduce c- fos, SP expression in the central nervous system related.