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内皮素 1(ET 1)可浓度依赖性的抑制大鼠肺动脉平滑肌细胞 (PASMCs)电压门控钾电流 (IKV) [1] ,但ET 1对PASMCs电压门控钾通道 (KV)基因表达的调节目前尚少见报道 ,本组研究拟从mRNA和蛋白水平 ,阐明内皮素受体拮抗剂BQ12 3对PASMCsKV1 5表达的影响。材料与方法 PASMCs的分离、
Endothelin 1 (ET 1) inhibits voltage-dependent potassium current (IKV) in rat pulmonary artery smooth muscle cells (PASMCs) in a concentration-dependent manner [1], but the regulation of voltage-gated potassium channel (KV) gene expression in PASMCs by ET 1 At present, it is seldom reported that this study intends to elucidate the effect of endothelin receptor antagonist BQ12 3 on the expression of KV1 5 in PASMCs from the mRNA and protein levels. Materials and methods PASMCs separation,