溴敌隆是近年来发现最有价值的第二代抗凝血杀鼠剂之一,国内现已合成。本文报道国产溴敌降致突变作用的研究结果。实验方法 1.Ames试验:①点试法:试验菌株为组氨酸营养缺陷型鼠伤寒沙门氏菌TA_(100)、TA_(1535)、TA_(98)、TA_(1537)及TA(1538)5种,前4种菌株的阳性对照物分别为甲基磺酸甲酯、亚硝基胍,正定霉素及9-氨基亚啶,用量分别为每平皿2μl及2、5、10μg。溴敌隆所用剂量为每平皿10μg。②标准平 Bromadiolone is one of the most valuable second-generation anticoagulant rodenticides found in recent years and is now synthesized in China. This article reports the results of the study on the mutagenic effects of brominated domestic enemies. Experimental methods 1.Ames test: ①Test method: The test strains were 5 kinds of TA (100), TA 1535, TA 98, TA 1537 and TA 1538 of histidine auxotrophic Salmonella typhimurium , The positive control of the first four strains were methyl methanesulfonate, nitrosoguanidine, n-amidocin and 9-amino-sub-pyridine, respectively, the amount of 2μl per plate and 2, 5, 10μg. The dose of bromadiolone is 10 μg per plate. ② standard flat