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胆酸及蛇胆汁经硫酸溶液脱水反应，均在紫外区３８７±１ｎｍ波长处产生最大吸收。用紫外分光光度法测定９种１０批蛇胆中总胆酸以胆酸计的含量，结果满意。本法适用于蛇胆的质量分析。
The dehydration reaction of cholic acid and snake bile juice with sulfuric acid solution produces maximum absorption in the ultraviolet region at a wavelength of 387±1 nm. Ultraviolet spectrophotometry was used to determine the content of total cholic acid in 9 batches of 10 batches of snake gall of cholic acid. The results were satisfactory. This law applies to the quality analysis of snake gall.