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白细胞介素4(IL-4)具有多种免疫效应,为深入研究IL-4在体内对T细胞分化和Ig血清型产生、转换的调节作用,该文用基因转移的方法使IL-4在小鼠体内长期表达,并探讨了IL-4在体内的免疫调节作用.先将小鼠IL-4cDNA片段插入逆转录病毒载体HindⅢ酶切部位,用包装细胞进行包装,将经射线处理的包装细胞与骨髓细胞共育48小时,再将感染后的骨髓细胞注入IgE低反应性C57BL/6小鼠体内.数月(3～8月)后对用上述方法构建
Interleukin-4 (IL-4) has a variety of immune effects. In order to further study the regulatory effect of IL-4 on T cell differentiation and Ig serotype production and transformation in vivo, IL- In vivo and in vivo.Mice IL-4 cDNA fragment was inserted into the retroviral vector Hind Ⅲ digestion site, packaged with packaging cells, the radiation-treated packaging cells And bone marrow cells incubated for 48 hours, then the infected bone marrow cells into IgE low reactivity in C57BL / 6 mice in vivo. Month (3 ~ August) after the above method to construct