采用ＲＴ－ＰＣＲ对广东地区３３例肝炎病人血清进行ＨＧＶＲＮＡ检测，结果２例（６．１％）阳性。对其中１株输血后ＨＧＶ（ＣＨ－３）基因组５＇端非翻译区（５＇ＵＴＲ）进行分子克隆与测序，并分别与Ｌｉｎｎｅｎ等报道的２株ＨＧＶ（ＰＮＦ２１６１，Ｒ１０２９１）和Ｌｅａｒｙ等报道的ＧＢＶ－Ｃ相比较，其核苷酸序列的同源性分别为８９．２％、８８．７％、８７．１％。本研究首次证实我国华南地区存在ＨＧＶ感染。ＨＧＶ高度保守区５＇ＵＴＲ的分子克隆与核苷酸序列的测定对开展ＨＧＶ感染的基因诊断具有重要意义。 HGV RNA was detected by RT-PCR in 33 patients with hepatitis in Guangdong Province, and the results were positive in 2 cases (6.1%). The 5 ’untranslated region (5’UTR) of HGV (CH-3) genome was cloned and sequenced after being transfused from one of the strains. The two strains were respectively reported by two strains of HGV (PNF2161, R10291) and Leary reported by Linnen et al GBV-C, the homologies of nucleotide sequences were 89.2%, 88.7% and 87.1% respectively. This study for the first time confirmed the existence of HGV infection in southern China. Molecular cloning and nucleotide sequencing of 5 ’UTR of highly conserved region of HGV are of great significance for genetic diagnosis of HGV infection.