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为了观察牛磺酸转运体(transporter,TAUT)的cDNA在大鼠脑内的分布,用35S标记的TAUTDNA探针,对大鼠全脑连续切片进行原位分子杂交,阳性反应广泛分布于脑灰质的不同区域,例如嗅球、梨状皮质、大脑皮质(2~3层)、齿状回、海马、下丘脑(室旁核、视上核、弓状核、背内侧核、腹内侧核等等)、丘脑网状核、内侧缰核、小脑皮质Purkinje细胞层和颗粒细胞层(Purkinje细胞为阴性,而周围的胶质细胞为阳性)、脑干的某些核(孤束核、三叉神经脊束核和延髓腹外侧区),在灰质的神经元和神经胶质细胞观察到反应颗粒。在脑的白质(例如:嗅束、视神经、视交叉、视束、前连合、穹窿、内囊、终纹、胼胝体、内侧丘系、外侧丘系、锥体束)的胶质细胞内也有反应颗粒。
In order to observe the distribution of cDNA of taurine transporter (TAUT) in rat brain, in situ serial hybridization of serial sections of rat whole brain was performed with 35S labeled TAUTDNA probe. The positive reaction was widely distributed in brain gray matter Such as the olfactory bulb, piriform cortex, cerebral cortex (layers 2 to 3), dentate gyrus, hippocampus, hypothalamus (paraventricular nucleus, supraoptic nucleus, arcuate nucleus, dorsomedial nucleus, ventral medial nucleus, etc.) ), The thalamic reticular nucleus, the medial habenular nucleus, the cerebellum Purkinje cell layer and the granulosa cell layer (Purkinje cells are negative and the surrounding glial cells are positive), some of the brainstem nucleus (nucleus tractus solitarius, trigeminal nerve ridge The nucleus accumbens and rostral ventrolateral medulla) were observed in gray matter neurons and glial cells. There are also glial cells in the white matter of the brain (eg, olfactory tract, optic nerve, optic chiasm, optic tract, anterior commissure, vault, internal capsule, terminal vein, corpus callosum, medial mound, lateral cumulus, pyramidal tract) Reaction particles.