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采用标准微电极技术和膜片钳记录技术，分别研究了粉防己碱（Ｔｅｔ）和颅通定（ｒｏｔｕｎｄｉｎｅ）对家兔窦房结动作电位及豚鼠心室肌单细胞钙电流的影响。结果表明：Ｔｅｔ１～２００μｍｏｌ·Ｌ－１，ｒｏｔｕｎｄｉｎｅ３～３００μｍｏｌ·Ｌ－１能浓度依赖性地降低兔窦房结动作电位的ＡＰＡ，Ｖｍａｘ和ＳＰ４，延长ＳＣＬ。Ｔｅｔ０．３μｍｏｌ·Ｌ－１与ｒｏｔｕｎｄｉｎｅ１μｍｏｌ·Ｌ－１合用亦有作用。在膜片钳实验中Ｔｅｔ０．１μｍｏｌ·Ｌ－１和ｒｏｔｕｎｄｉｎｅ１μｍｏｌ·Ｌ－１合用能有效抑制豚鼠心室肌单细胞钙电流，抑制率为１９．２％。提示，两种植物源性抗钙剂有良好的协同效果。
The effects of tetrandrine and rotundine on the action potentials of sinus node and single cell calcium in guinea pig ventricular myocytes were studied using standard microelectrode technique and patch-clamp recording technique. The results showed that Tet1 ~ 200μmol·L-1, rotundine3 ~ 300μmol·L-1 could decrease the APA, Vmax and SP4 of the sinus node action potential in a concentration-dependent manner and prolong the SCL. Tet0.3μmol·L-1 and rotundine1μmol·L-1 also play a role. In the patch-clamp experiment, Tet0.1μmol·L-1 and rotundine1μmol·L-1 could effectively inhibit the single-cell calcium current in guinea pig ventricular myocytes with a inhibition rate of 19.2%. Tip, two plant-derived anti-calcium have a good synergistic effect.