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目的探讨实验性单侧前牙反修复体对大鼠髁突软骨中甲状旁腺激素相关蛋白(PTHrP)及PTH/PTHrP受体-1(PTH1R)表达的影响。方法在6周龄SD大鼠左侧上、下切牙粘接金属不良修复体,使其呈反关系,2、4、8周后取颞下颌关节(TMJ),苏木精-伊红(HE)染色观察组织形态学变化,免疫组织化学染色及实时定量聚合酶链反应检测PTHrP、PTH1R的表达情况。结果 8周时,实验组髁突软骨出现明显退行性变,PTHrP阳性细胞较对照组显著增多(P<0.01),PTH1R阳性细胞明显减少(P<0.01);实验组髁突软骨PTHrP mRNA的表达显著高于对照组(P<0.01),而PTH1R mRNA表达明显降低(P<0.01)。结论髁突软骨PTHrP的高表达因PTH1R的低表达不能有效发挥作用,可能是髁突软骨骨关节病样变的重要分子机制之一。
Objective To investigate the effect of experimental unilateral anterior inverted arch prosthesis on the expression of parathyroid hormone related protein (PTHrP) and PTH / PTHrP receptor -1 (PTH1R) in rat condylar cartilage. METHODS: The left and right inferior incisors of 6-week-old SD rats were used to attach bad metallic prostheses. The relationship between TMJ, TMJ and hematoxylin-eosin HE staining and histopathological changes were observed. The expressions of PTHrP and PTH1R were detected by immunohistochemical staining and real - time quantitative polymerase chain reaction. Results After 8 weeks, the condylar cartilage in the experimental group showed obvious degenerative changes, PTHrP positive cells increased significantly (P <0.01) and PTH1R positive cells decreased significantly (P <0.01). The expression of PTHrP mRNA in condylar cartilage of experimental group (P <0.01), while PTH1R mRNA expression was significantly lower (P <0.01). Conclusion The high expression of PTHrP in condylar cartilage may not be effective due to the low expression of PTH1R, which may be one of the important molecular mechanisms of condylar cartilage and osteoarthrosis.