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目的 探讨白介素 1β(IL 1β)对精氨酸加压素(AVP)诱导的新生SD大鼠心脏成纤维细胞 (CF)胶原合成的影响。方法 采用3H 脯氨酸掺入法 ,观察IL 1β对AVP诱导的CF胶原合成的影响。结果 ( 1)在基础状态下 ,1×10 5U/LIL 1β组CF3H 脯氨酸掺入率为 ( 94 7± 2 1 4)cpm/ 5× 10 3 细胞 ,较对照组〔( 16 5 7± 31 2 )cpm/ 5× 10 3 细胞〕显著降低 (P <0 0 5 ) ;( 2 )CF3H 脯氨酸掺入率随AVP作用浓度的增加而增高 ,其中 10 7,10 6mol/LAVP组的CF3H 脯氨酸的掺入率分别为 :( 5 41 3± 95 8和 5 6 5± 72 4)cpm/ 5×10 3 细胞 ,明显高于对照组〔( 16 5 7± 31 2 )cpm/ 5× 10 3 细胞 ,P均 <0 0 1〕 ;( 3)IL 1β可以浓度依赖的方式抑制 10 7mol/LAVP诱导的CF3H 脯氨酸掺入率 ,其中 1× 10 5U/LIL 1β +AVP组和 2× 10 5U/LIL 1β +AVP组CF3H 脯氨酸的掺入率分别为 :( 2 71 3± 42 2和 2 19 7± 37 1)cpm/ 5× 10 3 细胞 ,较AVP组明显降低 (分别为P <0 0 5 ,P <0 0 1)。结论IL 1β可抑制基础状态下和AVP诱导的CF合成胶原 ,对逆转高血压病心肌肥厚可能有一定的作用
Objective To investigate the effect of interleukin-1β (IL-1β) on arginine vasopressin (AVP) -induced cardiac fibroblast (CF) collagen synthesis in neonatal SD rats. Methods 3H proline incorporation method was used to observe the effect of IL 1β on AVP-induced CF collagen synthesis. Results (1) Under the basal condition, the incorporation rate of CF3H proline in 1 × 10 5 U / LIL 1β group was (94 7 ± 2 1 4) cpm / 5 × 10 3 cells, compared with that in the control group (P <0.05). (2) The incorporation rate of proline in CF3H increased with the increase of the concentration of AVP, in which 10 7,10 6 mol / L AVP group The incorporation rate of CF3H proline was (5 41 3 ± 95 8 and 5 6 5 ± 72 4) cpm / 5 × 10 3 cells, which was significantly higher than that of the control group [(167 ± 31 2) cpm / 5 × 10 3 cells, all P <0.01). (3) IL-1β could inhibit 10 7 mol / L AVP-induced incorporation of CF3H proline in a concentration-dependent manner, in which 1 × 10 5 U / LIL 1β + AVP group And 2 × 10 5 U / LIL 1β + AVP group, the incorporation rates of CF3H proline were (2 71 3 ± 42 2 and 2 19 7 ± 37 1) cpm / 5 × 10 3 cells, which were significantly lower than that of AVP group (P <0 05, P <0 01, respectively). Conclusion IL 1β can inhibit collagen synthesis induced by AVP and CF in the basal state and may play a role in reversing cardiac hypertrophy in hypertensive patients