论文部分内容阅读
目的探讨不同剂量和不同疗程米非司酮对大鼠子宫异位内膜基质金属蛋白酶-9(MMP-9)及其组织特异性抑制物-1(TIMP-1)表达的影响。方法 65只子宫内膜异位症大鼠随机分为低剂量组、高剂量组、对照组。各个剂量组分别灌胃20、30、40 d后取异位子宫内膜组织用免疫组织化学法检测MMP-9及TIMP-1的表达,并计算MMP-9/TIMP-1比值。结果低剂量组和高剂量组均能使异位内膜MMP-9表达下降(P<0.05),TIMP-1表达升高(P<0.05),高剂量组的作用更加明显,与低剂量组差异有统计学意义(P<0.05);用药30 d低剂量组TIMP-1表达最高,与用药20 d和40 d相比差异有统计学意义(P<0.05),同样高剂量组用药30d TIMP-1的表达也最高,与用药20 d和用药40 d比较差异有统计学意义(P<0.05)。与用药20、40 d比较差异有统计学意义(P<0.05)。结论米非司酮能够降低大鼠异位子宫内膜的侵袭能力,高剂量米非司酮抑制效果更明显;用药30 d米非司酮对大鼠异位内膜的侵袭能力抑制最强,延长用药时间不能使异位内膜侵袭能力继续下降。
Objective To investigate the effects of mifepristone on the expression of matrix metalloproteinase-9 (MMP-9) and its tissue inhibitor of tissue inhibitor-1 (TIMP-1) in rat uterus at different doses and different courses of treatment. Methods 65 endometriosis rats were randomly divided into low dose group, high dose group and control group. The ectopic endometrium tissues were taken from the rats in each dose group for 20, 30 and 40 days respectively. The expressions of MMP-9 and TIMP-1 were detected by immunohistochemistry and the ratio of MMP-9 / TIMP-1 was calculated. Results Both low dose group and high dose group could decrease the expression of MMP-9 in ectopic endometrium (P <0.05) and increase the expression of TIMP-1 (P <0.05), and the effect of high dose group was more obvious than that of low dose group (P <0.05). The expression of TIMP-1 in the low dose group for 30 days was the highest, which was significantly different from that on the 20th and 40th day (P <0.05) -1 expression was also the highest, compared with the medication for 20 d and the drug for 40 d, the difference was statistically significant (P <0.05). Compared with the medication for 20 and 40 d, the difference was statistically significant (P <0.05). Conclusion Mifepristone can reduce the ectopic endometrial invasion of rats, the high dose of mifepristone inhibitory effect is more obvious; medication 30 mifepristone inhibition of ectopic endometrium strongest inhibition, Prolong the medication time can not make ectopic endometrial invasion continues to decline.