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目的对尿液特征组分与糖尿病早期肾损害的关系进行了初步探索。方法对2011年12月-2012年5月间28例2型糖尿病组、33例2型糖尿病肾病组及26例健康对照组尿液中尿蛋白含量和几种常见非蛋白氮物质,包括肌酸、尿囊素、肌酐、尿酸和假尿嘧啶核苷的浓度进行测定,采用多种归一化方法对数据进行对比分析,并通过t检验减少高效液相色谱测定的变量信息,保留P<0.05的检出峰进行主成分分析,获得分类结果。结果采用体积归一化方法,发现健康对照组尿液中肌酸、尿囊素和尿酸的含量与2型糖尿病组和糖尿病肾病组相比,差异均有统计学意义(P<0.05),2型糖尿病组尿液中尿蛋白的浓度与糖尿病肾病组相比,差异有统计学意义(P<0.05)。结论通过肌酸、尿囊素、尿酸和尿蛋白的联合测定为肾脏损伤程度的监测及疗效观察提供依据,为2型糖尿病患者肾功能损坏的早期预防与诊断进行初步判断提供了新的方法。
Objective To explore the relationship between urine components and early diabetic nephropathy. Methods Urine proteinuria and several common non-protein nitrogen substances, including creatine, in urine of 28 patients with type 2 diabetes mellitus and 33 patients with type 2 diabetic nephropathy and 26 healthy controls from December 2011 to May 2012 were analyzed. , Allantoin, creatinine, uric acid and pseudouridine were determined by a variety of normalized methods to compare the data, and by t-test to reduce high-performance liquid chromatography determination of the variable information, P <0.05 Of the detected peak principal component analysis to obtain the classification results. Results The urine volume of creatine, allantoin and uric acid in healthy control group were significantly lower than those in type 2 diabetes mellitus group and diabetic nephropathy group (P <0.05), and 2 Compared with diabetic nephropathy group, urinary proteinuria in diabetic group had significant difference (P <0.05). Conclusion The combined determination of creatine, allantoin, uric acid and urinary protein provides the basis for the monitoring of renal injury and the observation of the curative effect. It provides a new method for the early diagnosis and prevention of renal damage in type 2 diabetic patients.