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目的:探讨参附注射液(SFI)对缺氧缺血性脑损伤(HIBD)新生大鼠脑组织中半胱氨酸蛋白酶-1(Caspase-1)蛋白和mRNA的表达。方法:制备新生大鼠HIBD模型,将新生7日龄Sprague-Dawley(SD)大鼠随机分为假手术组(S)、生理盐水对照组(C)、参附组(SF),每组按术后观察时间点不同进一步分为3 h、12 h、24 h、6天、14天5个亚组,采用RT-PCR方法检测病变侧大脑皮层Caspase-1 mRNA的表达,用免疫组织化学法行Caspase-1免疫阳性细胞计数。结果:C组和SF组Caspase-1 mRNA和Caspase-1免疫阳性细胞计数在24 h、6天、14天均较S组升高(P<0.01)。24 h开始增加,6天达到高峰,随后开始下降,但14天仍高于S组(P<0.01);SF组Caspase-1 mRNA和免疫阳性细胞计数在24 h、6天、14天较C组减少(P<0.01)。结论:参附注射液(SFI)下调HIBD新生大鼠Caspase-1蛋白和mRNA的表达水平,SFI对新生大鼠HIBD有保护作用。
Objective: To investigate the effect of Shenfu Injection (SFI) on the expression of caspase-1 protein and mRNA in the brain of neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods: HIBD model was established in neonatal rats. Sprague-Dawley (SD) rats of 7 days old were randomly divided into sham operation group (S), saline control group (C) and Shenfu group (SF) The time points after operation were further divided into 3 subgroups: 3 h, 12 h, 24 h, 6 d, 14 d and 5 subgroups. The expression of Caspase-1 mRNA was detected by RT-PCR and immunohistochemistry Caspase-1 immunoreactive cells were counted. Results: The counts of Caspase-1 mRNA and Caspase-1 immunoreactive cells in group C and group SF were higher than those in group S at 24 h, 6 d and 14 d (P <0.01). (P <0.01). The counts of Caspase-1 mRNA and immunopositive cells in SF group were 24 h, 6 d and 14 d respectively, compared with those in group C Group decreased (P <0.01). Conclusion: Shenfu Injection (SFI) can down-regulate the expression of Caspase-1 protein and mRNA in neonatal rats with HIBD, and SFI has a protective effect on HIBD in neonatal rats.