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目的建立获得高纯度ＨＳＣ／ＨＰＣ细胞群的方法，用于研究造血干／祖细胞（ＨＳＣ／ＨＰＣ）的功能与生物学特性。方法利用免疫磁珠分选法（ＭＡＣＳ）从新鲜脐血和骨髓穿刺液中富集ＣＤ３４＋细胞，再用荧光激活细胞分选术（ＦＡＣＳ）分选纯化ＣＤ３４＋／ＣＤ３８－和ＣＤ３４＋／ＣＤ３８＋细胞亚群。结果ＭＡＣＳ分选的ＣＤ３４＋细胞群纯度可达９５％；ＦＡＣＳ分选的ＣＤ３４＋／ＣＤ３８－和ＣＤ３４＋／ＣＤ３８＋两细胞亚群纯度均可达９９％以上。结论联合使用ＭＡＣＳ和ＦＡＣＳ可在数小时内大量纯化ＨＳＣ／ＨＰＣ及其中重要细胞亚群。
Objective To establish a method for obtaining high-purity HSC/HPC cell population for studying the functional and biological characteristics of hematopoietic stem/progenitor cells (HSC/HPC). METHODS: CD34+ cells were enriched from fresh umbilical cord blood and bone marrow aspirate by immunomagnetic bead sorting (MACS), and then CD34+/CD38- and CD34+/CD38+ subpopulations were sorted by fluorescence-activated cell sorting (FACS). . Results The purity of CD34+ cell population with MACS sorting was up to 95%. The purity of CD34+/CD38- and CD34+/CD38+ subpopulations with FACS sorting was up to 99%. Conclusion The combination of MACS and FACS can be used to massively purify HSC/HPC and its important cell subpopulations within a few hours.