新生儿的细胞免疫状态如何,与成人有何异同之处,这些对决定新生儿疾病的防治有重要参考价值。由于Ficoll分离淋巴细胞需血量较大,且新生儿取血较难,加上传统的荧光显微镜计数费工耗时,新生儿T淋巴细胞亚群的分析少有报道。我们用流式细胞仪分析了微量全血OKT染色的新生儿T细胞亚群20例,观察了正常新生儿各T亚群的均值、变异及与正常成人的差异。 Neonatal cellular immune status, and adults what are the similarities and differences between these decisions on the prevention and treatment of neonatal diseases have important reference value. As Ficoll separation of lymphocytes required greater blood volume, and neonatal blood collection more difficult, coupled with the traditional fluorescence microscopy time-consuming, neonatal T lymphocyte subsets analysis rarely reported. We used flow cytometry analysis of 20 cases of whole blood OKT stained neonatal T cell subsets, observed the normal newborn T subpopulations of the average, mutation and differences with normal adults.