聚丙烯酰胺等电聚焦电泳(IEF)分辨率很高,但所用试剂昂贵。采用薄层和超薄层IEF可大大降低实验费用并获得常规2mm胶板无法获得的优点。一般制备超薄胶是将凝胶与玻璃板共价连接、使能承受电泳后的染色、脱色处理,但有关试剂国内不易得到。我们按实际条件摸索了薄层和超薄层胶板的制备方法并用以对一些蛋白质样品进行了分析比较。 Polyacrylamide isoelectric focusing electrophoresis (IEF) high resolution, but the reagents used expensive. The use of thin and ultra-thin layers of IEF reduces the cost of experimentation and the benefits that conventional 2mm glues can not. The general preparation of thin plastic gel is covalently connected with the glass plate, to withstand electrophoresis after dyeing, bleaching treatment, but the domestic reagents is not easy to get. Based on the actual conditions, we explored the preparation methods of thin and ultra-thin plastic sheets and used them to analyze and compare some protein samples.