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利用纳米石墨、单链脱氧核糖核酸开发了一个新型的荧光生物传感器并使用脱氧核糖核酸酶作为信号放大器对溶液中的汞离子进行检测.在最佳实验条件下,这种新型荧光生物传感器对汞离子的检出限达到0.5 nmol/L,比传统未经信号放大的传感器低20倍.得益于汞离子(Hg2+)可以结合两个胸腺嘧啶碱基(T)形成强力且稳定的T-Hg2+-T复合结构(T-Hg2+-T)的作用,该传感器对汞离子有着出色的选择性.此新型荧光生物传感器有望成为未来检测其他金属离子和生物分子的新工具.“,”A nano-graphite–DNA hybrid sensor as a cheap, effective fluorescent sensor for detection of mercury( II) ions in aqueous solution was presented. Furthermore, an amplification strategy based on nano-graphite for Hg2+detection by using DNase I was demonstrated. The proposed amplified assay was simple and cost-effective with a limit of detection ( LOD ) for Hg2+of 0. 5 nmol/L, which was about 20-fold lower than that of traditional unamplified homogeneous assays without amplification. The selectivity of the sensor for Hg2+ is outstanding due to the high specificity of T-Hg2+-T formation. The fluorescent sensor might be the new tool for other metal ions or biomolecules.