论文部分内容阅读
通过荧光猝灭法和同步荧光光谱法对头孢哌酮钠与牛转铁蛋白的相互作用机理进行了研究.结果表明,两种方法用相同的公式计算得到的二者的猝灭机理、结合位点,相互作用力以及协同作用基本一致.并且通过对Δλ = 60 nm 和λex = 295 nm 的数据进行比较,得出同步荧光光谱法较之于传统荧光光谱法有更高的灵敏度和准确度.因此,同步荧光光谱法极大地丰富了小分子配体与蛋白之间相互作用机理的研究的方法.“,”The reaction mechanism of cefoperazone sodium to bovine transferrin was investigated by both fluorescence quenching and synchronous fluorescence spectroscopy. Quenching mechanism, binding site,interaction and cooperativity were obtained from those two methods using the same equation and the results were consistent. According to the data obtained from Δλ = 60 nm and λex = 295 nm,it indicated that the synchronous fluorescence spectroscopy has higher sensitivity and accuracy compared with conventional fluorescence spectroscopy. Therefore, synchronous fluorescence spectroscopy has greatly enriched the methods of mechanism research about interaction of smallmolecule ligands and protein binding.