AIM: To cloneΔ12 -fatty acid desaturase gene of Mortierella isabellina and to functionally characterize this gene in vitro and in vivo. METHODS: Reverse transcriptional polymerase chain reaction (RT-PCR) was used to clone the open reading frame ofΔ12-fatty acid desaturase gene (D12D) of Mortierella isabellina. Plasmids pEMICL12 and pYMICL12 were constructed with it. pEMICL12 was transformed into Escherichia coli (E.coli) strain BL21 using CaCl2 method for expression after induction with IPTG. pTMICL12 was transformed into Saccharomyces cerevisiae strain INVSc1 using lithium acetate method for expression under the induction of galactose. Northern blotting method was used to investigate the effect of temperature on the transcriptional level of this gene in S.cerevislae strain IN-VScl. RESULTS: Recombinant plasmids pEMICL12 and pT-MICL12 were successfully constructed and transformed into E.coli and S.cerevisiae separately with appropriate method. After induction with IPTG and galactose, it was found that expression ofΔ12-fatty acid desaturase genes in E.coli and 5. cerevisiae under appropriate conditions led to the production of active A12-fatty acid desaturase, which could convert 17.876% and 17.604% of oleic acid respectively to linoleic acid by GC-MS detection in vitro and in vivo. CONCLUSION: Cloning and expression of M.isabellina D12D gene in E.coli and S.cerevisiae is successfully completed. AIM: To clone Δ12 -fatty acid desaturase gene of Mortierella isabellina and to functionally characterize this gene in vitro and in vivo. METHODS: Reverse transcriptional polymerase chain reaction (RT-PCR) was used to clone the open reading frame of Δ12-fatty acid desaturase gene (D12D) of Mortierella isabellina. Plasmids pEMICL12 and pYMICL12 were constructed with it. PEMICL12 was transformed into Escherichia coli (E. coli) strain BL21 using CaCl2 method for expression after induction with IPTG. PTMICL12 was transformed into Saccharomyces cerevisiae strain INVSc1 using lithium acetate method for expression under the induction of galactose. Northern blotting method was used to investigate the effect of temperature on the transcriptional level of this gene in S. cerevisiae strain IN-VScl. RESULTS: Recombinant plasmids pEMICL12 and pT-MICL12 were successfully constructed and transformed into E. coli and S. cerevisiae separately with an appropriate method. After induction with IPTG and galactose, it was found that expression of Δ12-fatty acid desaturase genes in E. coli and 5. cerevisiae under appropriate conditions led to the production of active A12-fatty acid desaturase, which could convert 17.876% and 17.604% of oleic acid respectively to linoleic acid by GC-MS detection in vitro and in vivo. CONCLUSION: Cloning and expression of M. isabellina D12D gene in E. coli and S. cerevisiae is successfully completed.