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本文合成了一种新的双核稀土配合物Na4Eu2L2.3NaNO3.12H2O[L=(2,6-bis[bis(carboxylatomethyl)amino]methyl-4-nitrophenolate),以下缩写为NO2-HXTA],并用红外光谱(IR)、元素分析(EA)等手段对其结构进行了表征。用凝胶电泳实验研究了该双核稀土配合物与pBR322 DNA的作用,发现在37℃时与DNA的切割作用不明显;但在50℃,pH7.2时,配合物在较低浓度(50μmol/L)下即可将70%的超螺旋DNA(CCC带)转化为缺刻产物(OC带);此外,用光谱学手段研究了双核稀土配合物与小牛胸腺(CT)DNA的作用,结果表明,配合物的加入使CTDNA的最大吸收峰(λmax=260 nm)增色和蓝移,并且使溴化乙锭EB-DNA复合物体系的荧光强度减弱。
In this paper, a novel dual-core rare earth complex Na4Eu2L2.3NaNO3.12H2O [2,6-bis [carboxy (carboxylatomethyl) amino] methyl-4-nitrophenolate has been synthesized, which is abbreviated as NO2-HXTA] (IR), elemental analysis (EA) and other means of its structure were characterized. The effect of the binuclear rare earth complex and pBR322 DNA was studied by gel electrophoresis and found that the effect of DNA cleavage was not obvious at 37 ℃. However, at 50 ℃ and pH 7.2, L), 70% of supercoiled DNA (CCC band) can be transformed into nicked product (OC band). In addition, the effect of binuclear rare earth complex on calf thymus (CT) DNA was studied by spectroscopy. , The addition of the complex to the maximum absorption peak of CTDNA (λmax = 260 nm) color and blue shift, and the ethidium bromide EB-DNA complex system decreased fluorescence intensity.