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目的建立饮水型大鼠氟斑牙模型,为深入研究氟性骨损伤发病机制提供科学依据。方法采用饮水加氟的方法复制大鼠氟斑牙模型,染氟剂量采用日测体重然后按体重(mg/kg)给予及尿氟、血氟监测的方法进行,尿氟、血氟采用微量氟法测定,血清中碱性磷酸酶(ALP)采用全自动生化分析仪测定,氟斑牙采用数码相机拍照,按照氟斑牙观测标准进行诊断及分度。结果 90 d末,高、中剂量组大鼠均出现明显氟斑牙,尿氟、血氟及血ALP含量与对照组相比均明显升高(P<0.05)。染氟剂量与尿氟水平显著相关(r=0.924,P=0.038);染氟剂量与血氟水平呈显著相关(r=0.948,P=0.026);高、中剂量组大鼠氟负荷水平明显高于对照组;氟斑牙发生率与染氟剂量呈正相关(r=0.983,P=0.017)。结论染氟剂量与氟斑牙的发生存在明显的剂量-反应关系:16 mg/kg和32 mg/kg的染氟剂量,14 d即可发生Ⅰ度氟斑牙,35 d即可发生典型氟斑牙。
Objective To establish a drinking water rat model of dental fluorosis to provide a scientific basis for further study of the pathogenesis of fluorosis. Methods The model of dental fluorosis was duplicated by drinking water and fluoride. The dosage of fluoride was measured by daily body weight and then by body weight (mg / kg) and urine fluoride and blood fluoride monitoring. Determination of serum alkaline phosphatase (ALP) using automatic biochemical analyzer, dental fluorosis using a digital camera to take photographs, in accordance with the diagnostic criteria for dental fluorosis diagnosis and indexing. Results At the end of 90 d, the levels of ALP in the high and middle dose groups were significantly higher than those in the control group (P <0.05). The fluoride dose was significantly correlated with urinary fluoride level (r = 0.924, P = 0.038). There was a significant correlation between fluoride dose and blood fluoride level (r = 0.948, P = 0.026) The incidence of dental fluorosis was positively correlated with the fluoride dose (r = 0.983, P = 0.017). Conclusion There is a significant dose-response relationship between fluoride dose and the occurrence of dental fluorosis: fluorine dose of 16 mg / kg and 32 mg / kg can develop grade I dental fluorosis at 14 days, and typical fluoride can occur at 35 days Sting.