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目的模拟临床上血糖控制不佳出现的糖浓度波动，研究波动性细胞外高糖浓度（ＦＥＨＧ）对肾小球系膜细胞（ＭｓＣ）增殖及其纤维连接蛋白（Ｆｎ）合成的影响。方法利用四甲基偶氮唑蓝（ＭＩＴ）比色法和竞争抑制ＥＬＩＳＡ法动态观察不同葡萄糖浓度及波动性高糖浓度对ＡｓＣ增殖和ＦＮ合成的作用。结果高糖浓度抑制ＭｓＣ增殖，此抑制作用和糖浓度成正相关，同时刺激ＭｓＣ的ＦＮ合成增加；ＦＥＨＧ也抑制ＭｓＣ的增殖，且对ＦＮ的合成有较强的刺激作用。结论细胞外高葡萄糖环境抑制ＭｓＣ增殖，且呈浓度依赖性；波动性细胞外高葡萄糖环境抑制ＭｓＣ增殖，且抗增殖作用大于持续性高葡萄糖浓度组；细胞外高葡萄糖环境和波动性细胞外高葡萄糖均增加ＭｓＣＦｎ的合成，其作用机制尚待进一步研究，波动性高糖浓度对ＭｓＣ的Ｆｎ合成的刺激作用可能在治疗不充分的糖尿病（ＤＭ）患者出现糖尿病肾病（ＤＮ）起重要作用。
Objective To simulate the fluctuating sugar concentration in poorly controlled blood glucose levels and to investigate the effects of fluctuating high extracellular glucose concentration (FEHG) on the proliferation and fibronectin (Fn) synthesis of mesangial cells (MsC). Methods MTT assay and competition inhibition ELISA were used to observe the effects of different glucose concentrations and fluctuating high glucose concentrations on AsC proliferation and FN synthesis. Results The high concentration of glucose inhibited the proliferation of MsC. The inhibitory effect was positively correlated with the concentration of glucose and increased the FN synthesis of MsC. FEHG also inhibited MsC proliferation and stimulated the synthesis of FN. Conclusion Extracellular high glucose environment inhibits MsC proliferation in a concentration-dependent manner. Vascular extracellular high glucose environment suppresses MsC proliferation and its antiproliferative effect is higher than that of persistent high glucose concentration group. Extracellular high glucose environment and fluctuating extracellular high Glucose both increase the synthesis of MsCFn. The mechanism of action is still to be further studied. The stimulatory effect of fluctuating high concentration of glucose on Fn synthesis of MsC may play an important role in the treatment of diabetic nephropathy (DN) in patients with inadequate diabetes mellitus (DM).