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背景:诸多研究认为血管内皮型一氧化氮合酶(endothelialnitricoxidesynthase,eNOS)基因多态性与心、脑、肾疾病存在相关性,但eNOS-Glu298Asp与中国老年人心肌梗死的关系是否相关尚不明确。设计:以诊断为依据的病例对照研究。目的:探讨老年人血管内皮型一氧化氮合酶基因Glu298Asp多态性的分布,及其与老年心肌梗死的关系。地点、对象和方法:37例老年心肌梗死患者为病例组,均为南京医科大学第一附属医院门诊及住院患者,其中20例既往无高血压史者为病例亚组;172例本院体检中心复检者(对照组),其中92例既往无高血压者为对照亚组,分别检测病例组和对照组的身高、体质量、空腹血脂等指标,采用聚合酶链反应(polymerasechainreaction,PCR)和限制性片断长度多态性(restrictionfragmentlengthpolymorphism,RFLP)检测eNOS基因Glu298Asp多态性。主要观察指标:4组老年人eNOS基因Glu298Asp多态性及298Asp等位基因频率。结果:病例组Glu/Asp基因型高于对照组(32.43%和18.02%),两组eNOS基因Glu298Asp多态性构成差异有显著性意义(χ2=3.87,P<0.05)。病例亚组Glu/Asp基因型(35.00%)高于对照亚组(10.87%),差异有显著性意义(χ2=7.43,P<0.01)。病例组298Asp等位基因频率高于对照组,差异无显著性意义(P>0.05)。病例亚组298Asp等
BACKGROUND: Many studies suggest that the genetic polymorphisms of endothelial nitric oxide synthase (eNOS) are associated with heart, brain and kidney diseases. However, whether eNOS-Glu298Asp is associated with myocardial infarction in Chinese elderly remains unclear . Design: A case-control study based on diagnosis. Objective: To investigate the distribution of Glu298Asp polymorphism of vascular endothelial nitric oxide synthase gene in the elderly and its relationship with senile myocardial infarction. Location, Subjects and Methods: Thirty-seven elderly patients with myocardial infarction were selected as the case group. All of them were outpatients and inpatients of the First Affiliated Hospital of Nanjing Medical University. Twenty patients with no prior history of hypertension were the case subgroup. Another 172 patients (Control group). Among 92 cases who had no prior hypertension, the control subgroups were included in this study. The body height, body weight, fasting blood lipids and other indexes of the case group and the control group were detected respectively. Polymerase chain reaction (PCR) and Restriction fragment length polymorphism (RFLP) detection of eNOS gene Glu298Asp polymorphism. MAIN OUTCOME MEASURES: The eNOS gene Glu298Asp polymorphism and 298 Asp allele frequency in the four groups of elderly people. Results: The genotypes of Glu / Asp in case group were higher than those in control group (32.43% vs 18.02%). There was significant difference in Glu298Asp polymorphism of eNOS gene between two groups (χ2 = 3.87, P <0.05). The Glu / Asp genotype (35.00%) in case subgroup was higher than that in control subgroup (10.87%), the difference was significant (χ2 = 7.43, P <0.01). The frequency of 298Asp allele in case group was higher than that in control group, with no significant difference (P> 0.05). Case subgroup 298Asp and so on