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本实验用白色家兔,在常温(20±1℃)和高温环境(40±1℃)时,分别记录动物的直肠温度,并分别从动物的小脑延髓池抽取脑脊液(C. S. F. ),从耳缘静脉采血,用蛋白结合法测脑脊液(C. S. F. )和血浆中(cAMP)含量。动物在常温(对照组)和高温环境(热应激组)的平均直肠温度相比较,两者有显著性差异。而动物在常温及热应激时C. S. F. 和血浆中cAMP含量其差异均不显著。表明热应激时体温虽有显著升高而不能引起C. S. F. 和血浆中cAMP含量的增加。说明既往实验发热时体温升高很可能是由于C. S. F. 中cAMP含量增加所致。
In this experiment, the white rectum was used to record the rectal temperature of animals at normal temperature (20 ± 1 ℃) and high temperature (40 ± 1 ℃) respectively. Cerebrospinal fluid (CSF) Venous blood was collected for determination of cerebrospinal fluid (CSF) and plasma (cAMP) content by protein binding assay. The average rectal temperature of animals at room temperature (control group) and high temperature environment (heat stress group) was significantly different. However, there was no significant difference in the content of cAMP between C. S. F. and plasma at normal temperature and heat stress. Indicating that although heat was significantly elevated body temperature can not cause C. S. F. and plasma cAMP content increased. This suggests that the increase in body temperature during previous experimental fever is most likely due to an increase in cAMP in C. S. F.