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为研究人子宫内膜、早孕蜕膜单层细胞及其条件培养液对早期胚胎发育的作用，本文应用酶消化法将人增殖晚期早分泌期子宫内膜及早孕蜕膜消化成单个细胞或细胞团块，分别在体外培养，并冷冻保存。建立单层细胞并制成条件培养液，由于人胚取材较困难，此文采用小鼠早期胚胎共培养，结果显示，人子宫内膜及早孕蜕膜在体外发育良好，并可冷冻保存，复苏率＞ ４０％ 。小鼠胚胎的卵裂率、桑椹胚及囊胚形成率明显高于对照组（Ｐ＜ ０．００１），早孕蜕膜组较子宫内膜组略高，但无显著性差异。两种细胞的条件培养液均可刺激胚胎发育，但无显著性差异，而胚泡形成率在早孕蜕膜组明显高于对照组（Ｐ＜ ０．０５）。提示：人子宫内膜及早孕蜕膜细胞能释放某些刺激胚胎发育的物质有利于胚胎发育
In order to study the role of human endometrium, decidual monolayer in early pregnancy and conditioned medium on early embryonic development, we digested human endometrium and early pregnancy decidual cells into single cells or cells by enzymatic digestion Clumps, were cultured in vitro, and cryopreserved. The establishment of monolayer cells and made of conditioned medium, the human embryo obtained more difficult, this article using mouse early embryo co-culture, the results show that human endometrium and early pregnancy decidua developed well in vitro and cryopreservation, recovery Rate> 40%. The cleavage rate, morula and blastocyst formation rate in mouse embryos were significantly higher than those in control group (P <0.001). The decidualization group in early pregnancy was slightly higher than that in endometrium group, but there was no significant difference. The conditioned medium of both cells could stimulate embryonic development, but no significant difference, while the blastocyst formation rate in early decidual group was significantly higher than the control group (P <0.05). Tip: human endometrium and early pregnancy decidual cells can release some substances that stimulate embryonic development conducive to embryonic development