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以甘蓝型油菜９５Ｚ１９２的组培小孢子植株为供试材料，以移栽前在试管培养过程中加倍、移栽时浸根加倍、移栽后花期滴腋芽加倍共３种方法的１４个处理进行油菜小孢子植株的加倍试验。结果显示：移栽时浸根加倍效果最好，植株成活率和加倍率最高。其中０．０５％秋水仙碱＋１．５％二甲基亚砜混和液浸根４ｄ的处理植株成活率９１．３０％、加倍率９３．６５％，效果最好；浸根２ｄ的处理植株成活率９５．４５％，加倍率８０．９５％，效果次之。且试验方法简单，花费时间短，为有效的加倍方法。同时，通过考种分析了加倍处理对小孢子植株农艺性状的影响。
Tissue culture microspore plants of Brassica napus 95Z192 were used as test materials, doubled in tube culture before transplanting, doubled in rooting during transplanting, and 14 treatments of 3 methods of transplanting axillary buds in flowering stage after transplanting Double test of microspore plants in rapeseed. The results showed that the best doubled effect was when the roots were transplanted, and the survival rate and doubling rate of the plants were the highest. The survival rate of the treated plants treated with 0.05% colchicine + 1.5% DMSO solution for 4 days was 91.30%, and the doubling rate was 93.65% Rate of 95.45%, double rate of 80.95%, the effect of the second. And the test method is simple, take a short time, as effective double method. At the same time, we analyzed the effect of doubling treatment on the agronomic characters of microspore plants.