Neptunea cumingii is an important nutrition-rich economic species in China. Juveniles of N . cumingii suffer from high mortality at low temperatures, which has proved a limiting factor in raising seedlings in artificial habitats. Previous research has shown that N . cumingii displays aggregation behavior in response to adverse environmental changes. Therefore, we determined the effects of temperature, food, size of juvenile snails, substratum type, and density of juvenile snails on the aggregation behavior of N . cumingii . Results show that, at a low (4℃) or a high (22℃) temperature, juvenile snails adjusted to the inhospitable environment by exhibiting increased aggregation behavior. However, their aggregation behaviors differed at these two temperatures. There was no significant difference in the aggregation rate, but the typical aggregation size was larger at 4℃ than at 22℃. At 10℃ or 16℃, aggregation behavior of juvenile snails reduced. Aggregation increased in the satiation treatment at 10℃ and 16℃. Small-sized juveniles tended to have higher aggregation rates (92.22％) and a larger typical aggregation size. More juveniles were distributed in the bottom of shaded substrata. A larger typical aggregation size or higher density significantly reduced the mortality of juvenile snails at a low temperature (4℃). These results broaden our understanding of gastropod aggregation behavior and can be used to develop and improve commercial breeding strategies and resource recovery for N . cumingii .