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应用建立的Fab’放射免疫测定方法检测了166例病毒性肝炎患者血清层粘连蛋白（Laminin，LN）水平。结果60例急性肝炎、49例慢性迁延性肝炎（CPH）、34例慢性活动性肝炎（CAH）和23例肝硬化患者血清LN分别为114．74±2．34、110．17±32．18、144．84±38．90及171．13±40．41μg/L，仅CAH和肝硬化病例较正常对照116．64±19．85μg／L显著升高，且有从急性肝炎→慢性肝炎→肝硬化发展，血清LN逐步升高趋势。血清LN水平与血清透明质酸、Ⅳ型胶原显著正相关，而与常规肝功能指标A／C、ALT、Bil不相关。但A／G≥1．5的患者LN水平显著较A／G＜1．5的患者低。根据ROC曲线分析，以160μg／L为临界值诊断肝硬化，灵敏度69．6％、特异性84．3％，准确性81．1％。因此血清LN的检测对肝纤维化程度判断，尤其肝硬化的诊断有一定价值。
The level of serum Laminin (LN) in 166 patients with viral hepatitis was detected by Fab ’radioimmunoassay. Results Serum LN in 60 patients with acute hepatitis, 49 patients with chronic persistent hepatitis (CPH), 34 patients with chronic active hepatitis (CAH) and 23 patients with cirrhosis was 114.74 ± 2.34 and 110.17 ± 32.18, respectively , 144.84 ± 38.90 and 171.13 ± 40.41μg / L, respectively. Only CAH and cirrhosis cases were significantly higher than the 116.64 ± 19.85μg / L normal control group, and from acute hepatitis → chronic hepatitis → Liver cirrhosis development, serum LN gradually increased. Serum LN levels were positively correlated with serum hyaluronic acid and type Ⅳ collagen, but not with conventional liver function indexes A / C, ALT and Bil. However, patients with A / G≥1.5 had significantly lower LN levels than those with A / G <1.5. According to the ROC curve analysis, the diagnosis of cirrhosis with a threshold of 160 μg / L had a sensitivity of 69.6%, a specificity of 84.3% and an accuracy of 81.1%. Therefore, the detection of serum LN to determine the degree of liver fibrosis, especially in the diagnosis of cirrhosis of the liver has a certain value.