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我们在1978年搞水稻有性杂交先是在植株的自然条件下,从杀雄、授粉到培育,直到收获种子,都是在田间进行的。这种方法,很受外界自然条件影响,其结果花工大,效率低。我们做的200多个组合,只有70多个组合结实,结实率仅有百分之十几。 鉴于上述情况,我们初次试验用离体培养法搞水稻有性杂交,并对稻穗离体培养液的氮、磷、钾的含量做了选择配方,共做杂交组合6个,47穗,收杂交种子229粒,平均结实率达32.7％(见表一)。杂交种籽粒饱满,经发芽鉴定出苗正常,现初报如下:
In 1978, we carried out sexual hybridization in rice plants under the natural conditions, from the killing, pollination to cultivation, until the harvest of seeds, are carried out in the field. This method, very affected by the natural conditions of the outside world, the result of large flower workers, low efficiency. We made more than 200 combinations, only more than 70 combinations strong, seed set rate of only a dozen percent. In view of the above, we first conducted sexual hybridization of rice by in vitro culture and selected the content of nitrogen, phosphorus and potassium in in vitro culture medium of rice earwax, making a total of 6 hybrids and 47 spikes 229 hybrid seeds, the average seed set rate of 32.7% (see Table I). Hybrid seeds full, germination identified germination normal, are reported as follows: