Objective This study aimed to investigate the genetic background of mitochondrial genes in young patients with Coronary heart disease(CHD) to provide a foundation for the early prevention of young patients with CHD.Methods 115 cases of young(≤ 45 years) CHD Chinese Han patients(case group),100 cases of older(> 45 years) Chinese Han CHD patients(experimental group) hospitalized and 100 cases of healthy people through physical examination(control group) at the General Hospital of PLA between January 2014 and December 2015 were selected.General information,clinical assessment,pedigree analysis,and mitochondrial full sequence scanning were performed.The pedigrees of one patient harbouring the C5263 T mutation were recruited.Mitochondrial functional analysis including cellular reactive oxygen species(ROS) levels and mitochondrial membrane potential(MMP) were performed on pedigrees with the C5263 T mutation(mutation group) and without the mutation(non-mutation group).Results The differences in biochemical tests(P > 0.05) between the case group and experimental group were not significant.The C5263 T single-nucleotide mutation of the mitochondrial ND2 gene was observed in 2 young CHD patients in the case group.The premature CHD of these 2 patients followed a pattern of maternal inheritance.The mutation group(Ⅰ1,Ⅱ2) had higher ROS levels(4750.82 ± 1045.55 vs.3888.58 ± 487.60,P = 0.022) and lower MMP levels(P = 0.045) than the non-mutation group(Ⅱ1,Ⅲ1,Ⅲ2).Conclusion We speculated that the mitochondrial C5263 T mutation might be associated with the occurrence CHD in Chinese Han young people. Objective This study aimed to investigate the genetic background of mitochondrial genes in young patients with Coronary heart disease (CHD) to provide a foundation for the early prevention of young patients with CHD. Methods 115 cases of young (≤ 45 years) CHD Chinese Han patients Chinese Han CHD patients (experimental group) hospitalized and 100 cases of healthy people through physical examination (control group) at the General Hospital of PLA between January 2014 and December 2015 were selected . General information, clinical assessment, pedigree analysis, and mitochondrial full sequence scanning were performed. The pedigrees of one patient harboring the C5263 T mutation were recruited. Mitochondrial functional analysis including cellular reactive oxygen species (ROS) levels and mitochondrial membrane potential (MMP) were performed on pedigrees with the C5263 T mutation (mutation group) and without the mutation (non-mutation group). Results The difference s in biochemical tests (P> 0.05) between the case group and experimental group were not significant. The C5263 T single-nucleotide mutation of the mitochondrial ND2 gene was observed in 2 young CHD patients in the case group. The premature CHD of these 2 patients had a pattern of maternal inheritance. The mutation group (Ⅰ1, Ⅱ2) had higher ROS levels (4750.82 ± 1045.55 vs.3888.58 ± 487.60, P = 0.022) and lower MMP levels Ⅱ1, Ⅲ1, Ⅲ 2) .Conclusion We speculated that the mitochondrial C5263 T mutation might be associated with the occurrence CHD in Chinese Han young people.