利用戊二醛交联法将辣根过氧化物酶标记的福氏志贺氏菌抗体固定在多壁碳纳米管修饰的四通道丝网印刷碳电极表面,制备了可用于检测福氏志贺氏菌的酶免疫传感器。采用循环伏安法对不同修饰电极进行电化学表征。根据抗原-抗体特异性结合形成的免疫复合物使敏感膜有效扩散截面积减小的特性,采用循环伏安法检测样品中的福氏志贺氏菌。在优化的实验条件下,该酶免疫传感器对福氏志贺氏菌的检测范围为104~109 cfu/mL,检出限为3.4×103 cfu/mL(S/N=3)。该酶传感器还具有较好的特异性、重现性、稳定性和准确性。 The horseradish peroxidase-labeled Shigella flexneri antibody was immobilized on the surface of carbon nanotube modified four-channel screen-printed carbon electrode by glutaraldehyde cross-linking method. Corynebacterium enzyme immunoassay sensor. Cyclic voltammetry was used to characterize the different modified electrodes. According to the antigen-antibody-specific immune complex formed by the sensitive membrane effective diffusion cross-sectional area reduced characteristics, the use of cyclic voltammetry samples Shigella flexneri. Under the optimal experimental conditions, the detection range of Shigella flexneri was 104-109 cfu / mL with the detection limit of 3.4 × 103 cfu / mL (S / N = 3). The enzyme sensor also has better specificity, reproducibility, stability and accuracy.