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本研究组既往的研究表明，备用根猫脊髓背角组织块和提取液促神经突起生长的活性明显增强。为观察去传入猫脊髓背角提取液对神经元存活的影响，我们采用单侧后肢备用根猫（切除一侧Ll－L。、L，－Sz背报节，保留L。背根为备用根）五只，分别取术后五天T12-S3节段脊髓背角组织，匀浆、离心获得上清液、制成条件培养液后，加入鸡胚背根节神经细胞是液培养。于48h时，以l％台盼蓝染色，计数神经细胞存活率。结果显示：手术侧组神经细胞存活率为0.85±0.04，而非手术侧组神经细胞存活率仅为0．55fo．10。两者相比有显著性差异（P＜0．of）。提示备用根猫脊髓背角提取液对培养中的鸡胚背报节神经元除有促进神经突起生长的作用外还有明显的促神经元存活作用。
Previous studies in our group showed that the activity of neurite outgrowth in the spinal cord dorsal horn tissue and extract of Spared Root Cattle increased significantly. In order to observe the influence of the extracted extract of cat spinal cord dorsal horn on the survival of neurons, we used unilateral hindlimb backup root cat (Ll-L., L, -Sz back section of the excision side, Roots) five, were taken five days after T12-S3 segment spinal dorsal horn tissue, homogenate, centrifuged to obtain the supernatant, made of conditioned medium, adding chicken embryo dorsal root ganglion cells is liquid culture. At 48h, l% trypan blue staining, counting of nerve cell survival. The results showed that the survival rate of neurons in the lateral operation group was 0.85 ± 0.04, while the survival rate of the non-surgical lateral group was only 0.55fo. 10. There was a significant difference between the two (P <0.of). Tip spare root cat spinal dorsal horn extract cultured chick embryo nocturnal neurons in addition to promote the role of neurite growth but also significantly promote the survival of neurons.