建立高效液相色谱法测定注射用他唑巴坦钠 /哌拉西林钠的含量。采用 C1 8柱 ,以甲醇 -水 - 10 %氢氧化四丁基铵溶液 (45 0 :5 35 :15 ,用磷酸调节 p H至 3.5 )为流动相 ,检测波长为 2 30 nm,线性范围分别为哌拉西林 0 .0 8～ 1.6 mg/ ml,他唑巴坦 0 .0 2～ 0 .2 m g/ m l,回收率分别为 99.7% ,10 0 .1% ,RSD分别为 0 .8% ,0 .9% (n=6 ) To establish a high performance liquid chromatography for the determination of tazobactam sodium injection / piperacillin sodium content. The mobile phase consisted of C1 8 column with methanol-water-10% tetrabutylammonium hydroxide solution (45 0: 5 35:15, pH = 3.5 with phosphoric acid) as mobile phase at a detection wavelength of 230 nm and linear range For piperacillin 0. 08 ~ 1.6 mg / ml, tazobactam 0.2 ~ 0.2 mg / ml, the recovery rates were 99.7%, 100.1%, RSD were 0.8% , 0 .9% (n = 6)