论文部分内容阅读
目的研究改良白膜法汇集浓缩少白细胞血小板质量,为今后开展汇集浓缩血小板的制备提供依据。方法采用改良白膜法制备汇集浓缩血小板56袋检测各项质量指标,并随机抽取56袋单采血小板样本作比对;将37袋汇集浓缩血小板于贮存期d1(即采集当天)、d2、d3分别取样,用白细胞滤器进行过滤,测定不同贮存时间、过滤前后血小板膜表面糖蛋白分子CD62P和PAC-1的表达率;随机抽取37袋单采血小板作比对。结果改良白膜法制备的汇集浓缩少白细胞血小板容积、血小板含量、Ph值、红细胞混入量等检测结果与单采血小板各质量指标间比较差异无统计学意义(P>0.05),其白细胞混入量远低于单采血小板中白细胞混入量两者间存在显著性差异(P<0.01),无菌试验均为阴性;汇集浓缩血小板贮存期3d内滤白前后血小板膜表面糖蛋白分子CD62P和PAC-1的表达率均无统计学意义(P>0.05);贮存期3d内汇集浓缩血小板及单采血小板膜表面糖蛋白分子CD62P和PAC-1的表达率也均无统计学意义(P>0.05)。结论改良白膜法汇集浓缩少白细胞血小板各项质量指标均达到混合浓缩血小板及单采血小板国家标准,血小板体外活性保持良好;血小板专用型白细胞滤器对贮存期3d内的汇集浓缩血小板进行滤白处理不会造成血小板的明显活化和损伤;血小板振荡仪(22±2)℃振荡保存3d的汇集浓缩(单采)血小板存在持续的活化损伤,但活化和损伤并未明显改变血小板的体外活性。
OBJECTIVE: To study the method of improving the white blood cell platelet by enriching white blood cells and providing the basis for the preparation of concentrated platelets. Methods A total of 56 bags of concentrated platelets were prepared by improved tunica albuginea method for detection of quality indicators. Fifty-six bags of apheresis platelets were randomly selected for comparison. Thirty-seven bags of concentrated platelets were collected for storage at d1 (day of collection), d2 and d3 Samples were collected and filtered with a leukocyte filter. The expression rates of CD62P and PAC-1 on platelet membrane surface were measured before and after filtration at different storage times. 37 bags of apheresis platelets were randomly selected for comparison. Results There was no significant difference in platelet volume, platelet content, PH value, mixed amount of erythrocytes, etc between the collected white blood cells and the quality indexes of apheresis platelets (P> 0.05). The leukocyte incorporation (P <0.01), and the sterility test was negative. The accumulation of platelet membrane glycoprotein molecules CD62P and PAC-6 on platelet membrane before and after storage for three days during the storage of concentrated platelet, (P> 0.05). The expression rates of CD62P and PAC-1 on concentrated platelets and apheresis platelets during storage period were also not statistically significant (all P> 0.05) . Conclusion The modified white blood cell platelet aggregation leukocyte platelet quality indicators reached the national standard of mixed platelet and apheresis platelets, platelet activity remained good in vitro; platelet-specific leukocyte filter white platelet enrichment within three days of storage of concentrated platelets were whitened No obvious activation and damage of platelets were observed. Platelet aggregation (apheresis) platelets with oscillatory preservation at plateau (22 ± 2) ℃ for 3 days had sustained activation injury, but activation and injury did not change the in vitro activity of platelets significantly.