Objective To determine the optimum extraction method and condition of polysaccharides from Trollius chinensis (TCP).Methods With the yield of TCP as index and water as solvent,reflux extraction,ultrasonic extraction and supercritical CO2 extraction were employed to determine the efficient extraction method of TCP.Moreover,the conditions of reflux extraction were optimized by single factor test and orthogonal test.The content of polysaccharide was measured with phenol-sulfuric acid method.Results The data displayed that the crude polysaccharide (CPS) yield of regular reflux extraction was (11.7±0.12)%,superior to ultrasonic extraction and supercritical CO2 extraction.The optimum conditions of reflux extraction were as follows:extraction temperature of 95 ℃,extraction time of 3 h and the liquid-solid ratio of 20∶1.Under these conditions,the polysaccharide yield was(1.45±0.03)%,which was well in close agreement with the predicted value.Conclusion Regular reflux extraction was regarded as the optimal extraction method of TCP.The optimum conditions of reflux extraction were as follows:extraction temperature of 95 ℃,extraction time of 3 h and the liquid-solid ratio of 20∶1.