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本文用8个不同冬性小麦品种分期播种子8月中旬、9月上旬和9月中旬，将其单株于10月下旬收集并保存于-4℃或-8℃环境下，11月份进行控制冻害试验，冻害速率为2℃／h，将难以区分的不耐冻品种贮存于-4℃。但在3月这些难以区分的不耐冻性品种则变得较易区分。贮存在-8℃下的幼苗耐冻性降低较-4-℃下的幼苗更为迅速。在11月播种的那些耐冻性差的品种中（Rose、Rita和Sioux-land），其耐冻性较耐冻性品种（Norstar）表现更差。在-8℃下，Rita和Siouxland第二和第三播期的幼苗于2月份死亡。除过Norstan和Alabaskaja表现最抗冻以外，其余品种都死于3月。通过-12℃和-15℃下幼苗贮存，能迅速地鉴定小麦、小黑麦、黑麦等基因型的优异抗寒性。
In this paper, eight different winter wheat varieties were sown in stages by mid-August, early September and mid-September. The plants were collected and stored at -4 ℃ or -8 ℃ in late October and controlled in November Frost damage test, frost damage rate of 2 ℃ / h, indistinct cold species will be indistinguishable storage at -4 ℃. But in March these indistinguishable varieties of non-freezing became easier to distinguish. Seedling freeze resistance stored at -8 ° C reduced more rapidly than seedlings at -4 ° C. Among those cultivars with low resistance to freezing (Rose, Rita and Sioux-land) sown in November, their freezing tolerance was worse than that of the Norstar. At -8 ° C, seedlings of the second and third sowing dates of Rita and Siouxland died in February. With the exception of Norstan and Alabaskaja which show the most resistance to freezing, the remaining species died in March. By -12 ℃ and -15 ℃ seedling storage, can quickly identify wheat, triticale, rye and other genotypes of excellent cold resistance.