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目的:设计操作简单、成本低、便携和易于实现自动化等特点的小型化现场病原体检测系统,实现在传染病疫情爆发现场对传染病病原体的快速检测。方法:设计一款封闭式病原体检测卡盒,可进行一体化病原体核酸检测,并创新性地采用3D打印技术对该卡盒进行迭代设计和制造。在此基础上,研发与之配套的全自动便携式核酸检测系统,整套系统可自动化完成病原体核酸的单重检测或多重检测。结果:在卡盒和全自动便携式核酸检测系统设计完成后,分别对其进行各项性能的测试,如卡盒的气密性和移液性能,混匀性能等,测试结果均能满足病原体核酸检测的要求。以百日咳杆菌为代表,将整套系统应用于实际的病原体核酸检测,实验结果表明该卡盒系统可自动化完成核酸检测。结论:该系统能够成功地完成现场传染病从样本输入到结果输出的自动化一体化检测过程,实验结果准确可靠。
OBJECTIVE: To design a miniaturized on-site pathogen detection system with simple operation, low cost, portability and easy automation so as to realize rapid detection of infectious pathogens at the outbreak of infectious disease outbreak. METHODS: A closed pathogen test cartridge was designed for the integrated detection of pathogen nucleic acids, and innovatively employed 3D printing technology to iteratively design and manufacture the cartridge. On this basis, R & D and its accompanying fully automated portable nucleic acid detection system, the entire system can be automated to complete pathogen nucleic acid single or multiple detection. Results: After the design of the cassette and the fully automatic portable nucleic acid detection system were completed, the performance of the cassette was tested respectively, such as the air-tightness and pipetting performance of the cassette, the mixing performance, etc. The test results could meet the needs of pathogen nucleic acid Testing requirements. Bordetella pertussis as the representative, the entire system is applied to the actual pathogen nucleic acid detection, the experimental results show that the cartridge system can be automated nucleic acid detection. Conclusion: The system can successfully complete the integrated testing process of in-situ infectious diseases from sample input to result output, the experimental results are accurate and reliable.