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骤冷组织的恒冷切片如不经固定,直接孵育,可出现酶的扩散,影响酶的定位,甚至出现假阴性反应。为加深对酶扩散的后果和聚乙烯吡咯烷酮(PVP)对防止酶扩散作用的认识,作者对胞液内的可溶性酶,LDH和G-6-PD在溶液中的扩散作用和聚乙烯吡咯烷酮的防扩散效果进行了观察。 取SD大鼠肝、肾及横纹肌组织,合包骤冷后,切片分组作如下处理:Ⅰ组在缓冲液内,37℃预处理10分钟;Ⅱ组缓冲液内加入PVP
Cold-frozen tissue sections such as not fixed, direct incubation, there may be enzyme diffusion, affecting the enzyme localization, and even false-negative reaction. In order to deepen the understanding of the consequences of enzyme diffusion and the role of polyvinylpyrrolidone (PVP) in preventing the spread of enzymes, the authors investigated the effects of soluble enzymes, the diffusion of LDH and G-6-PD in solution on cytosolic and polyvinylpyrrolidone Diffusion effect was observed. The liver, kidney and striated muscle tissues of SD rats were taken out. After the co-pacification, the slices were divided into the following groups: Group I was pretreated in buffer at 37 ℃ for 10 minutes; Group Ⅱ buffer was added with PVP